LOCUS       sequence-159516-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR reporter construct used for 
            microinjection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Kaymak E, Farley BM, Hay SA, Li C, Ho S, Hartman DJ, Ryder SP
  TITLE     Efficient generation of transgenic reporter strains and analysis of 
            expression patterns in Caenorhabditis elegans using Library MosSCI.
  JOURNAL   Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.24426.
  PUBMED    27294288
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2016 Jun 13. doi: 10.1002/dvdy.24426."
COMMENT     Sequence Label: pCFJ150-mbk2
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(84..108)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(598..618)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ctcctgggca caagacatgg gcagcgtgcc atcatcctgc gccgccaccg ccggcgggaa
       61 gccatggcta agtctagaca tggagcctgc ttttttgtac aaacttgtca ttctctgtct
      121 gaaacattca attgattatc gataaaatta attaagagag aatacaatga gaaaacggaa
      181 aatcgagcag gattttgggg aaagtcgggg aatttttgct atttaagctt tctttaacaa
      241 agaaactgca tttttccatg agattaccat gaaatttgcg ttaaatgggt gaatttataa
      301 catttacggc tagaaattaa ttttaaaaaa gagaataaac gaaaaattat tcgagaaaac
      361 gtctttgaag cagaaatgaa atgaaaaaac aaccgccagc gctgacaaaa atgatcaaac
      421 aaacggagta cggtatacgc ggtaagaccc aaatgaattt taattcggtg cgcctttaaa
      481 aaatgtattt ttttaatttc tttatccgtg ggcccaaatg tatttttttc ttgtggtttg
      541 atgtaaaaat tgggttatat tatttgtttt atggtttaat tttttaaaaa ctgatatcaa
      601 cttttctata caaagttgat agcttggcgt aatcatggtc atagctgttc ctgcctaggt
      661 gagctctggt accctctagt caaggcctta agtgagtcgt attacgtagc ccgggcctag
      721 ttctagacat tctctaatga aaaaatc
//
