LOCUS       sequence-159522-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas2B
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(470..517)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     513..531
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tcgccaaccc aagcaacggc gatcgccacg gtgcaagaca attgggacat ggccctagcc
       61 caacagccca acgaccccga aattttgatg gccctgggtt ttgtccatta cctcaatgca
      121 gattacgaag ccgccaatga tcgttggtat gaagccttag cggcggcaac gacccaggat
      181 ttccccccag ccctccaggc ggagatttat gcgggcctgg cgattagtat gtacgatttg
      241 gcccaaaggc aacccaccga cgttgaccag gaaattcttg tagggaaagc cgaaaaactc
      301 cgggcgatcg ccctagatct cgatcgttcc cagctcaacc cccgccgcct cgaacaaagt
      361 tggttatggt tccctgaggc gatcgccact tggcaaaccc tgctcgcttt agaagaatct
      421 ccctaggtca tatccggggc atacattcga tcaacttgag cgctctagac aaaaaacccg
      481 tcaagacccg tttagaggcc ccaaggggtt atgctagtta ttgctcagcg gtggcagcag
      541 ccaactcagc ttcctttcgg gctttgttag tcacctccta gctgactcaa atcaatgcgt
      601 gtttcataaa gaccagtgat ggattgatgg ataagagtgg catctaaaac ttcttttgta
      661 gacgtatatc gtttacgatc aattgttgta tcaaaatatt taaaagcagc gggagctcca
      721 agattcgtca acgtaaataa atgaataata ttttctgctt gttcacgtat tggtttgtct
      781 ctatgtttgt tatatgcact aagaacttta tctaaattgg catctgctaa aataacacgc
      841 ttagaaaatt cactgatttg ctcaataatc tcatctaaat aatgcttatg ctgctccaca
      901 aac
//
