LOCUS       sequence-159523-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacillus subtilis sgRNA expression vector; integrates into thrC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins 
            JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, 
            Brown ED, Qi LS, Huang KC, Gross CA
  TITLE     A Comprehensive, CRISPR-based Functional Analysis of Essential Genes
            in Bacteria.
  JOURNAL   Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. 
            Epub 2016 May 26.
  PUBMED    27238023
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.003"; journalName: "Cell"; date: "2016-06-2-
            2"; volume: "165"; issue: "6"; pages: "1493-506"
COMMENT     Sequence Label: pJMP3
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        854..929
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             950..979
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             995..1012
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 catgtaaaag atgaggttgg ttcattctcg aaaattacat ctgtgttctc agagcggggc
       61 gtgagctttg aaaaaatcct tcagctgcca attaaaggcc atgatgagtt agctgaaatc
      121 gtaattgtca cacatcatac atcagaagct gatttcagtg atatcctgca aaacctaaat
      181 gatttggaag tcgttcaaga agtcaaaagc acatatcgtg tagaagggaa cggttggagc
      241 taatgtggaa aggacttatc catcaatata aagaattttt acctgtaaca gatcaaacac
      301 cggcgctaac tttacatgaa ggaaacacac ctcttattca cctgccgaag ctgtctgagc
      361 agctcggaat tgagcttcat gtcaaaacgg aaggcgtcaa tcctacggga tcatttaaag
      421 atcgcggaat ggttatggct gtggcaaagg caaaagaaga aggcaatgac acgattatgt
      481 gcgcgtcaac aggtaacact tccgctgctg cggcagcata tgcagcccgt gctaacatga
      541 aatgcattgt catcatcccg aacggaaaaa ttgcatttgg aaaactcgct caagctgtca
      601 tgtacggagc cgagattatc gcaattgacg gaaactttga cgatgcgctt aaaattgtcc
      661 gttccatctg tgagaaatca ccgattgccc ttgtcaactc agtcaaccct taccgcattg
      721 aaggccaaaa aactgctgcc ttcggatcct agaagcttat cgaattcctt attaacgttg
      781 atataattta aattttattt gacaaaaatg ggctcgtgtt gtacaataaa tgtaactttc
      841 agtttagcgg tctgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
      901 aacttgaaaa agtggcaccg agtcggtgct ttttttgaag cttgggcccg aacaaaaact
      961 catctcagaa gaggatctga atagcgccgt cgaccatcat catcatcatc attgagtt
//
