LOCUS       sequence-159524-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas2B
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        281..315
                     /label=BBa_J23113
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.01"
     RBS             324..335
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(415..434)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gagcccctat aagatttttt ttgatactgt ggcggtctgt atttcccaga accttgaact
       61 ttttagacgg aaccttatat tcatcagtga tcaccgccca tccgacgcta tttgtgccga
      121 tagctaagcc tattgagtat ttcttatcca tggaattaat gtcgaacttg actttatgag
      181 ttgggagctc cttttgcaat cccacagtta actttatact ctatcactga tagggatgtc
      241 tgtctatcac tgatagggat attcctgctt ttttgttaac ctgatggcta gctcagtcct
      301 agggattatg ctagctacta gagaaagagg agaaatacta gatgtccaga ttagataaaa
      361 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa
      421 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa
      481 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact
      541 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta
      601 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag
      661 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac
      721 tagagaa
//
