LOCUS       sequence-159525-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas2A
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             47..58
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(67..101)
                     /label=BBa_J23113
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.01"
ORIGIN
        1 atgcgctgtt aatcacttta cttttatcta atctggacat ctagtatttc tcctctttct
       61 ctagtagcta gcataatccc taggactgag ctagccatca ggttaacaaa aaagcaggaa
      121 tatccctatc agtgatagac agacatccct atcagtgata gagtataaag ttaactgtgg
      181 gattgcaaaa ggagctccca actcataaag tcaagtagga gattaattcc atggataaga
      241 aatactcaat aggcttagct atcggcacaa atagcgtcgg atgggcggtg atcactgatg
      301 aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca
      361 aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa gcgactcgtc
      421 tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt tatctacagg
      481 agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga cttgaagagt
      541 cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga aatatagtag
      601 atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggtag
      661 attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat atgattaagt
      721 ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat gtggacaaac
      781 tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct attaacgcaa
      841 gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga cgattagaaa
      901 atctcattgc tcagctccc
//
