LOCUS       sequence-159536-         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pmex-5:GFP::H2B::PEST::cul-4 3'UTR reporter construct used for 
            microinjection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Kaymak E, Farley BM, Hay SA, Li C, Ho S, Hartman DJ, Ryder SP
  TITLE     Efficient generation of transgenic reporter strains and analysis of 
            expression patterns in Caenorhabditis elegans using Library MosSCI.
  JOURNAL   Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.24426.
  PUBMED    27294288
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2016 Jun 13. doi: 10.1002/dvdy.24426."
COMMENT     Sequence Label: pCFJ150-cul4
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(423..447)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aatgcccggg ggatcggtgg agctccaccg gtggcggccg ctccagaact agtccaccaa
       61 agccatctgc caagggagcc aagaaggccg ccaagaccgt cgttgccaag ccaaaggacg
      121 gaaagaagag acgtcatgcc cgcaaggaat cgtactccgt ctacatctac cgtgttctca
      181 agcaagttca cccagacacc ggagtctcct ccaaggccat gtctatcatg aactccttcg
      241 tcaacgatgt attcgaacgc atcgcttcgg aagcttcccg tcttgctcat tacaacaaac
      301 gctcaacgat ctcatcccgc gaaattcaaa ccgctgtccg tttgattctc ccaggagaac
      361 ttgccaagca cgccgtgtct gagggaacca aggccgtcac caagtacact tccagcaagt
      421 aaacccagct ttcttgtaca aagtggtaca taatatcatt tttattcctt tttcaaattc
      481 aatccttatc cgtgccactg gtctcttctc atctcgtttg ccctgttatt gaacagtttt
      541 cttcctttat ttgtgcgagt ttcgtctttt ttagagaaaa atgtactaaa tgttagtttc
      601 tgttttttca attatgtttt tctcttcacc taacatgtgc atccaaaata ctgttgaata
      661 catggttatt tgattgaatt ctttatacga aattcaactt t
//
