LOCUS       sequence-159539-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NowGFP gene for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Sarkisyan KS, Goryashchenko AS, Lidsky PV, Gorbachev DA, Bozhanova 
            NG, Gorokhovatsky AY, Pereverzeva AR, Ryumina AP, Zherdeva VV, 
            Savitsky AP, Solntsev KM, Bommarius AS, Sharonov GV, Lindquist JR, 
            Drobizhev M, Hughes TE, Rebane A, Lukyanov KA, Mishin AS
  TITLE     Green fluorescent protein with anionic tryptophan-based chromophore 
            and long fluorescence lifetime.
  JOURNAL   Biophys J. 2015 Jul 21;109(2):380-9. doi: 10.1016/j.bpj.2015.06.018.
  PUBMED    26200874
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2015.06"; journalName: "Biophys J"; date: 
            "2015-07-21- 21"; volume: "109"; issue: "2"; pages: "380-9"
COMMENT     Sequence Label: NowGFP /pQE-30
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..30
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             49..60
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(103..105,106..819)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEKLFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKMSLK
                     FICTTGKLPVPWPTLKTTLTWGMQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNAISGNANITADKQKNGIKA
                     YFTIRHDVEDGSVLLADHYQQNTPIGDGPVLLPDNHYLSTQSKQSKDPNEKRDHMVLLE
                     FVTAAGIPLGADELYK"
     primer_bind     complement(145..166)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(406..425)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     753..774
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      841..935
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 aaatttattt gctttgtgag cggataacaa tttcacacag aattcattaa agaggagaaa
       61 ttaactatga gaggatcgca tcaccagcac catcacggat ccgtgagcaa gggcgagaag
      121 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      181 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagatgtc cctgaagttc
      241 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcaaaaccac cctgacctgg
      301 ggcatgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      361 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      421 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      481 ggcgtcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacgccatc
      541 agcggcaacg ccaatatcac cgccgacaag cagaagaacg gcatcaaggc ctacttcacg
      601 atccgccacg acgtcgagga cggcagcgtg ctgctcgccg accactacca gcagaacacc
      661 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
      721 cagagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      781 gccgggatcc ctctcggcgc ggacgagctg tacaagtaaa agcttaatta gctgagcttg
      841 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
      901 tcggttgccg ccgggcgttt tttattggtg agaatccaag ctagcttggc gagattttca
      961 ggagctaagg aagctaaaat ggagaaaaa
//
