LOCUS       sequence-159541-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The first mutant of the Aequorea victoria green fluorescent protein 
            that forms a red chromophore..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Mishin AS, Subach FV, Yampolsky IV, King W, Lukyanov KA, Verkhusha 
            VV
  TITLE     The first mutant of the Aequorea victoria green fluorescent protein 
            that forms a red chromophore.
  JOURNAL   Biochemistry. 2008 Apr 22;47(16):4666-73. doi: 10.1021/bi702130s. 
            Epub 2008 Mar 27.
  PUBMED    18366185
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi702130s";
            journalName: "Biochemistry"; date: "2008-04-22- 22"; volume: "47"; 
            issue: "16"; pages: "4666-73"
COMMENT     Sequence Label: R10-3 /pQE-30
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(80..174)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(249..270)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     598..617
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     857..878
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
       61 tctcgccaag ctagcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
      121 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagct
      181 cagctccgca agcttgcggc cgctcacttg tacagctcgt ccatgccgag agtgatcccg
      241 gcggcggtca cgaactccag caggaccatg tgatcgcgtt tctcgttggg gtctttgctc
      301 agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      361 ggggtgttct gctggtagtg gtcggcgagc tgtacgctgc cgtcctcgag gttgtggcgg
      421 accttgaagt tcgcctggat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
      481 ctgttgtagt tgtactccaa cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      541 cccttcagct cgatgcggtt caccagggtg tcaccctcga acttcacctc ggcgcgggtc
      601 ttgtagttgc cgtcgtcctt gaagaatatg gtacgctcct ggacgtagcc ttcgggcatg
      661 gcggacttga agaagtcgtg ctgcttcatg tggtcagggt agcggctgaa gcactggtta
      721 ccgtagctca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
      781 atgagcttca gggtcagctt gccgtaggtg gcatcgccct cgccttcccc ggacacgctg
      841 aacttgtgac cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cc
//
