LOCUS       sequence-159544-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive CcaS and CcaR with gfpmut3 under the cpcG2-Delta-59 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Milias-Argeitis A, Rullan M, Aoki SK, Buchmann P, Khammash M
  TITLE     Automated optogenetic feedback control for precise and robust 
            regulation of gene expression and cell growth.
  JOURNAL   Nat Commun. 2016 Aug 26;7:12546. doi: 10.1038/ncomms12546.
  PUBMED    27562138
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-26"; volume: "7:12546. doi"; pages: " 
            10.1038/ncomms12546"
COMMENT     Sequence Label: pSKA413
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             919..942
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tttacgcatt atatggtgcc ttacgtgcct gtcccttaga taaagttaga atgcgatcct
       61 aacaaagtaa aattgaagaa aagggggaga aaatcgtaaa gaaatcggaa agttagctga
      121 tgcgactaaa actgttacat ttcgcaacat gaattaaccc agacctgttc accccttcag
      181 gtaagctgag gttgatagag aaaagcagga tgggctatga gaattctttt agtggaggat
      241 gatttgccgc tggcggaaac ccttgctgaa gcattgagtg accagcttta caccgttgat
      301 attgccaccg acgcttccct cgcctgggac tatgcctccc gactggaata tgacctcgtt
      361 attttggatg tgatgctgcc ggagttggac gggattaccc tctgtcaaaa atggcgatcg
      421 cacagttatt taatgccaat tttgatgatg acagccaggg atacgatcaa tgataaaatc
      481 acgggcttgg atgcgggggc ggatgattat gtggtcaagc cagtggattt gggggagtta
      541 tttgccaggg tgcgagcttt gttgcgtcgg ggttgtgcaa cgtgccaacc agttttagag
      601 tgggggccaa tcaggttgga tccaagcacc tatgaagtta gttatgacaa tgaggttttg
      661 tctttgaccc gcaaggaata cagcattctg gaattactac tccgcaatgg ccgtcgggtg
      721 ctaagtcgga gcatgattat cgatagtatc tggaagttgg agagtccccc agaggaagat
      781 acggttaagg tgcatgtgcg gagtttgcga caaaaattaa aaagtgccgg tttatcagca
      841 gatgccattg aaacggtcca tggcattggg tatcgtctgg ccaatttaac ggaaaaatct
      901 ttgtgccaag ggaaaaacga ctacaaggac gacgatgaca agtagggttt ttctactggg
      961 attcgctaat atatgggcaa att
//
