LOCUS       sequence-159545-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive CcaS and CcaR with gfpmut3 under the cpcG2-Delta-59 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Milias-Argeitis A, Rullan M, Aoki SK, Buchmann P, Khammash M
  TITLE     Automated optogenetic feedback control for precise and robust 
            regulation of gene expression and cell growth.
  JOURNAL   Nat Commun. 2016 Aug 26;7:12546. doi: 10.1038/ncomms12546.
  PUBMED    27562138
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-26"; volume: "7:12546. doi"; pages: " 
            10.1038/ncomms12546"
COMMENT     Sequence Label: pSKA413
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(43..145)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(175..222)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(226..249)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
       61 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
      121 gttggaacct cttacgtgcc gatcaacgtc tcattttcgc cagatatcga cgtccaaaaa
      181 acccctcaag acccgtttag aggccccaag gggttatgct agttacttgt catcgtcgtc
      241 cttgtagtca gctcgaggca aatggttata gcgttttaaa cagatagtaa ctgttgttcc
      301 ctggtcaact gcacttttta gcaagatact gccactgtgt aagtcaacac atttcttggc
      361 aaccattaaa ccgagtcctg ttcccgtaat atttctgaca tttttgcccc gatgaaaggg
      421 ttcaaaaatt tgcttttggt cctctggcga aatgccaatg ccctggtcgg tgacttcaaa
      481 aataatctgt tccgaatcta ggcttagggc aattttaatc tgccctcccc cgggagagta
      541 tttaatcgca ttagataaca gattagataa aatagaccgc actaaccttt catccaccaa
      601 tgccttcgta tcttgagcgc tacaaataaa gtcaaaataa tattggtcac tgacacttaa
      661 ttgaatttct tcgataaatt gctggaacaa taatttcaaa tctaaccaat taggattaaa
      721 ttccaatttg cccgcttcgg cacggttaat gattaaaata tcatccagga gctgtaccat
      781 atttttcacg gaattttgaa tacggtgtaa gttccggcta cgcttatcgg gatcaagcca
      841 ggccacttca gaattttcca gtaattgggc agcagctaag gccgtactga ggggagtacg
      901 aaattcatgg gaagccatgg agaaaaaacg agtttttagg cggcttaatt ctttttcccg
      961 ttctaaagct aggcggactt cctccgtgcg tcggcgc
//
