LOCUS       sequence-159549-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual Fluorescent endogenous miRNA sensor. Perfect complementary 
            region of hsa-miR-27a-3p in three copies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Lemus-Diaz N, Boker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M,
            Gruber J
  TITLE     Dissecting miRNA gene repression on single cell level with an 
            advanced fluorescent reporter system.
  JOURNAL   Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197.
  PUBMED    28338079
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-24"; volume: "7:45197. doi"; pages: " 
            10.1038/srep45197"
COMMENT     Sequence Label: p.UTA.2.0 miR-27a-3p 3 perfect copies.
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(132..845,846..848,849..851))
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(177..198)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     526..545
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     785..806
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(859..877)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acacacagat gtaatgaaaa taaagatatt ttattgcggc cagcggcctt cacagtggct
       61 aagttccgcc cggttcacag tggctaagtt ccgcccggtt cacagtggct aagttccgct
      121 cgagcgatcg cttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
      181 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
      241 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
      301 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagtgg
      361 gccttgatgc cgttcttctg cttgtcggcg gtgatataga cgttgtggct gatgtagttg
      421 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
      481 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
      541 tcgtccttga agaagatggt acgctcctgg acgtagcctt cgggcatggc ggacttgaag
      601 aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc ccaggtcagg
      661 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg
      721 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cctgtggccg
      781 tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc
      841 ttgctcacca ttggctagcc tatagtgagt cgtattaagt actctagcct taagagctgt
      901 a
//
