LOCUS       sequence-159552-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  No anti-scaffold control with 3xFLAG tags for Western blots. 
            Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH016
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..761
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             762..827
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 aaattatgag ctcatacatt cgtaagaaac gggcgcagga ggattagatg cgtaaaggcg
       61 aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc
      121 ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga
      181 agttcatctg tactactggt aaactgccgg ttccttggcc gactctggta acgacgctga
      241 cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca
      301 agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca
      361 cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc
      421 tgaaaggcat tgactttaaa gaagacggca atatcctggg ccataagctg gaatacaatt
      481 ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt
      541 ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac taccagcaaa
      601 acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg agcacgcaaa
      661 gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg gagttcgtaa
      721 ccgcagcggg catcacgcat ggtatggatg aactgtacaa agactacaag gaccatgacg
      781 gtgattacaa ggatcatgac atcgactaca aggatgacga tgacaagtag tgagaagctt
      841 gggcccgaac aaaaa
//
