LOCUS       sequence-159557-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual Fluorescent endogenous miRNA sensor. Perfect complementary 
            region of hsa-miR-19b-3p.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Lemus-Diaz N, Boker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M,
            Gruber J
  TITLE     Dissecting miRNA gene repression on single cell level with an 
            advanced fluorescent reporter system.
  JOURNAL   Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197.
  PUBMED    28338079
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-24"; volume: "7:45197. doi"; pages: " 
            10.1038/srep45197"
COMMENT     Sequence Label: p.UTA.2.0 miR 19b-3p
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(76..789,790..792,793..795))
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(121..142)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     470..489
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     729..750
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(803..821)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgtaatgaa aataaagata ttttattgcg gccagcggcc tgtgcaaatc catgcaaaac
       61 tgatcgagcg atcgcttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt
      121 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga
      181 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
      241 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
      301 gtgggccttg atgccgttct tctgcttgtc ggcggtgata tagacgttgt ggctgatgta
      361 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
      421 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
      481 gccgtcgtcc ttgaagaaga tggtacgctc ctggacgtag ccttcgggca tggcggactt
      541 gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccccaggt
      601 cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
      661 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacctgtg
      721 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
      781 gcccttgctc accattggct agcctatagt gagtcgtatt aagtactcta gccttaagag
      841 ctgtaattga actgggagtg gacacctgtg
//
