LOCUS       sequence-159561-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual Fluorescent endogenous miRNA sensor Control insert. Three 
            Perfect copies of complementary region of a siRNA control targeting 
            firefly luciferase encoded by the pGL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Lemus-Diaz N, Boker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M,
            Gruber J
  TITLE     Dissecting miRNA gene repression on single cell level with an 
            advanced fluorescent reporter system.
  JOURNAL   Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197.
  PUBMED    28338079
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-24"; volume: "7:45197. doi"; pages: " 
            10.1038/srep45197"
COMMENT     Sequence Label: p.UTA.2.0-GL-2-3 perfect copies
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     99..120
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(220..971)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
ORIGIN
        1 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
       61 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
      121 gatgggcacc accccggtga acagctcctc gcccttgctc accatgggcc cttcttaatg
      181 ttcttagggc tttaccaaca gtaccggaat gccaagcttt taagcgggtc gctgcagggt
      241 cgctcggtgt tcgaggccac acgcgtcacc ttaatatgcg aagtggacct gggaccgcgc
      301 cgccccgact gcatctgcgt gttcgaattc gccaatgaca agacgctggg cggggtttgt
      361 gtcatcatag aactaaagac atgcaaatat atttcttccg gggacaccgc cagcaaacgc
      421 gagcaacggg ccacggggat gaagcagctg cgccactccc tgaagctcct gcagtccctc
      481 gcgcctccgg gtgacaagat agtgtacctg tgccccgtcc tggtgtttgt cgcccaacgg
      541 acgctccgcg tcagccgcgt gacccggctc gtcccgcaga aggtctccgg taatatcacc
      601 gcagtcgtgc ggatgctcca gagcctgtcc acgtatacgg tccccatgga gcctaggacc
      661 cagcgagccc gtcgccgccg cggcggcgcc gcccgggggt ctgcgagcag accgaaaagg
      721 tcacactctg gggcgcgcga cccgcccgag tcagcggccc gccagttacc acccgccgac
      781 caaacccccg cctccacgga gggcgggggg gtgcttaaga ggatcgcggc gctcttctgc
      841 gtgcccgtgg ccaccaagac caaaccccga gccgcctccg aatgagagtg tttcgttcct
      901 tccccctccc cccgcgtcag acaaacccta accaccgctt aagcggcccc cgcgaggtcc
      961 gaagactcat tta
//
