LOCUS       sequence-159566-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Fujita T, Kitaura F, Oji A, Tanigawa N, Yuno M, Ikawa M, Taniuchi I,
            Fujii H
  TITLE     Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for 
            enChIP analysis.
  JOURNAL   Genes Cells. 2018 Feb 26. doi: 10.1111/gtc.12573.
  PUBMED    29480524
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells. 2018 Feb 26. doi: 10.1111/gtc.12573."
COMMENT     Sequence Label: 3xFLAG-dCas9/MSCV-EGFP
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(32..531)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(80..99)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     473..496
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(703..723)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcaccatgg tggcaagctt gggctgcagg tcgaaaggcc cggagatgag gaagaggaga
       61 acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc
      121 gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc cccggaccca ccccttccca
      181 gcctctgagc ccagaaagcg aaggagcaaa gctgctattg gccgctgccc caaaggccta
      241 cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag actagtgaga cgtgctactt
      301 ccatttgtca cgtcctgcac gacgcgagct gcggggcggg ggggaacttc ctgactaggg
      361 gaggagtaga aggtggcgcg aaggggccac caaagaacgg agccggttgg cgcctaccgg
      421 tggatgtgga atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg cccagcgggg
      481 ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc cggtagaatt
      541 cgtggccgct cgagtttaaa tagtacataa tggatttcct tacgcgaaat acgggcagac
      601 atggcctgcc cggttattat tatttttgac accagaccaa ctggtaatgg tagcgaccgg
      661 cgctcagctg gaattcgatc cggtagggat cgaacccttt cacaccttcc tcttcttctt
      721 ggggtcagcc ctgctgtctc caccgagctg agagaggtcg attcttgttt catagagccc
      781 cgtaattgac tgatgaatca
//
