LOCUS       sequence-159567-        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Fujita T, Kitaura F, Oji A, Tanigawa N, Yuno M, Ikawa M, Taniuchi I,
            Fujii H
  TITLE     Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for 
            enChIP analysis.
  JOURNAL   Genes Cells. 2018 Feb 26. doi: 10.1111/gtc.12573.
  PUBMED    29480524
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells. 2018 Feb 26. doi: 10.1111/gtc.12573."
COMMENT     Sequence Label: 3xFLAG-dCas9/MSCV-EGFP
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(56..58,59..61,62..775)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(56..58,59..61,62..772)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(101..122)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(362..381)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     709..730
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagcttg ccaccatggt
       61 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      121 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      181 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      241 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
      301 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      361 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      421 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      481 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
      541 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
      601 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      661 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      721 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg
      781 ccatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
      841 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat
      901 aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc
      961 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
     1021 ccagatgcgg tcccgccctc agcagtttct agagaaccat caga
//
