LOCUS       sequence-159570-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Fujita T, Kitaura F, Oji A, Tanigawa N, Yuno M, Ikawa M, Taniuchi I,
            Fujii H
  TITLE     Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for 
            enChIP analysis.
  JOURNAL   Genes Cells. 2018 Feb 26. doi: 10.1111/gtc.12573.
  PUBMED    29480524
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells. 2018 Feb 26. doi: 10.1111/gtc.12573."
COMMENT     Sequence Label: 3xFLAG-dCas9/MSCV-EGFP
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(230..250)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcggggctg ctaaagcgca tgctccagac tgccttggga aaagcgcctc ccctacccgg
       61 tagaattcgt ggccgctcga gtttaaatag tacataatgg atttccttac gcgaaatacg
      121 ggcagacatg gcctgcccgg ttattattat ttttgacacc agaccaactg gtaatggtag
      181 cgaccggcgc tcagctggaa ttcgatccgg tagggatcga accctttcac accttcctct
      241 tcttcttggg gtcagccctg ctgtctccac cgagctgaga gaggtcgatt cttgtttcat
      301 agagccccgt aattgactga tgaatcagtg tggcgtccag gacctccttt gtagaggtgt
      361 accgctttct gtctatggtg gtgtcgaagt acttgaaggc tgcaggcgcg cccaagttgg
      421 tcagagtaaa caagtggata atgttttctg cctgctccct gatgggctta tccctgtgct
      481 tattgtaagc agaaagcacc ttatcgaggt tagcgtcggc gaggatcact cttttggaga
      541 attcgcttat ttgctcgatg atctcatcaa ggtagtgttt gtgttgttcc acgaacagct
      601 gcttctgctc attatcttcg ggagaccctt tgagcttttc atagtggctg gccagataca
      661 agaaattaac gtatttagag ggcagtgcca gctcgttacc tttctgcagc tcgcccgcac
      721 tagcgagcat tcgtttccgg ccgttttcaa gctcaaagag agagtacttg ggaagcttaa
      781 tgatgaggtc ttttttgacc tctttatatc ctttcgcctc gagaaagtcg atggggtttt
      841 tttcgaagct tgatcgctcc atgattgtga tgcccagcag ttccttgacg cttttgagtt
      901 ttttagactt ccctttctcc actttggcca caaccagtac actgtaagcg actgtaggag
      961 aatcgaatcc gccgtatttc ttggggtccc aatctttttt gcgtgcgatc agcttgtcgc
     1021 tgttcct
//
