LOCUS       sequence-159577-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of redox sensitive GFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Hanson GT, Aggeler R, Oglesbee D, Cannon M, Capaldi RA, Tsien RY, 
            Remington SJ
  TITLE     Investigating mitochondrial redox potential with redox-sensitive 
            green fluorescent protein indicators.
  JOURNAL   J Biol Chem. 2004 Mar 26;279(13):13044-53. Epub 2004 Jan 13.
  PUBMED    14722062
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-03-26"; volume: "279(13)"; pages: "13044-53. 
            Epub 2004 Jan 13"
COMMENT     Sequence Label: pRSETB-ro2GFP
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             56..88
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(92..100,101..115)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(164..192)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 actttaagaa ggagatatac atatgcgggg ttctcatcat catcatcatc atggtatggc
       61 tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatcc
      121 ccccgctgaa ttcatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt
      181 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      241 tgcaacatac ggaaaactta cccttaaatt tatttccact actggaaaac tacctgttcc
      301 atggccaaca cttgtcacta ctttcactta tggtgttcaa tgcttttcaa gatacccaga
      361 tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
      421 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
      481 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
      541 tcttggacac aaattggaat acaactataa ctgccacaat gtatacatca tggcagacaa
      601 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
      661 tcaactagca gaccattatc aacaaaat
//
