LOCUS       sequence-159579-         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of redox sensitive GFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Hanson GT, Aggeler R, Oglesbee D, Cannon M, Capaldi RA, Tsien RY, 
            Remington SJ
  TITLE     Investigating mitochondrial redox potential with redox-sensitive 
            green fluorescent protein indicators.
  JOURNAL   J Biol Chem. 2004 Mar 26;279(13):13044-53. Epub 2004 Jan 13.
  PUBMED    14722062
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-03-26"; volume: "279(13)"; pages: "13044-53. 
            Epub 2004 Jan 13"
COMMENT     Sequence Label: pRSETB-ro3GFP
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..46
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             50..82
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(86..94,95..109)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(158..186)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 agaaggagat atacatatgc ggggttctca tcatcatcat catcatggta tggctagcat
       61 gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg atccccccgc
      121 tgaattcatg agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      181 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      241 atacggaaaa cttaccctta aatttatttc cactactgga aaactacctg ttccatggcc
      301 aacacttgtc actactttca gttatggtgt tcaatgcttt tcaagatacc cagatcatat
      361 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      421 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
      481 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
      541 acacaaattg gaatacaact ataactcaca ctgtgtatac atcatggcag acaaacaaaa
      601 gaatggaatc aaagttaact tca
//
