LOCUS       sequence-159580-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of redox sensitive GFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Hanson GT, Aggeler R, Oglesbee D, Cannon M, Capaldi RA, Tsien RY, 
            Remington SJ
  TITLE     Investigating mitochondrial redox potential with redox-sensitive 
            green fluorescent protein indicators.
  JOURNAL   J Biol Chem. 2004 Mar 26;279(13):13044-53. Epub 2004 Jan 13.
  PUBMED    14722062
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-03-26"; volume: "279(13)"; pages: "13044-53. 
            Epub 2004 Jan 13"
COMMENT     Sequence Label: pRSETB-ro3GFP
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(75..788)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHCVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLCTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(115..135)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     730..758
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(join(807..821,822..830))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(834..866)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(870..887)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggctttgtta gcagccggat caagcttcga attccatggt accagctgca gatctcgagc
       61 tcggatcctt attatttgta tagttcatcc atgccatgtg taatcccagc agctgttaca
      121 aactcaagaa ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag ggcagattgt
      181 gtgcacaggt aatggttgtc tggtaaaagg acagggccat cgccaattgg agtattttgt
      241 tgataatggt ctgctagttg aacgcttcca tcttcaatgt tgtgtctaat tttgaagtta
      301 actttgattc cattcttttg tttgtctgcc atgatgtata cacagtgtga gttatagttg
      361 tattccaatt tgtgtccaag aatgtttcca tcttctttaa aatcaatacc ttttaactcg
      421 attctattaa caagggtatc accttcaaac ttgacttcag cacgtgtctt gtagttcccg
      481 tcatctttga aaaatatagt tctttcctgt acataacctt cgggcatggc actcttgaaa
      541 aagtcatgcc gtttcatatg atctgggtat cttgaaaagc attgaacacc ataactgaaa
      601 gtagtgacaa gtgttggcca tggaacaggt agttttccag tagtggaaat aaatttaagg
      661 gtaagttttc cgtatgttgc atcaccttca ccctctccac tgacagaaaa tttgtgccca
      721 ttaacatcac catctaattc aacaagaatt gggacaactc cagtgaaaag ttcttctcct
      781 ttactcatga attcagcggg gggatcctta tcgtcatcgt cgtacagatc ccgacccatt
      841 tgctgtccac cagtcatgct agccatacca tgatgatgat gatgatgaga
//
