LOCUS       sequence-159588-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of tagged protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  TITLE     Barr-Gillespie TwinStrep Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CAPZA1-TwinStrepII
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..73
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     24..44
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    94..118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      119..128
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
       61 tataagcaga gctggtttag tgaaccgtca gatccaagtt tgtacaaaaa agcagggcgc
      121 caccatggcc gactttgagg atcgggtgtc ggatgaagag aaggtccgca tagccgctaa
      181 attcatcacc catgcacccc caggggagtt taatgaagta ttcaatgatg tccggctact
      241 gcttaacaat gacaatctcc tcagggaagg ggcagcacat gcatttgccc agtataacat
      301 ggatcagttc acacccgtga agatagaagg ctacgatgac caggtcttaa ttacagagca
      361 tggtgacctt ggtaacagca gatttttaga tccaagaaac caaatttcct ttaagtttga
      421 tcatttacgg aaagaagcaa gtgacccgca gccagaggat gtggatggag gcttgaagtc
      481 ttggagggag tcgtgtgaca gtgctctgag agcctatgtg aaagatcatt attccaacgg
      541 cttctgtact gtttacgcta aaactataga tgggcaacag accattattg catgtattga
      601 aagccaccag tttcaaccaa aaaacttctg gaatggtcgc tggagatcag agtggaagtt
      661 caccattaca ccaccttcag cccaggtggt tggagtactc aagattcagg ttcattatta
      721 tgaagatggc aatgttcagc tggttagtca taaagatgtg caggactcag taactgtttc
      781 taatgaaatc caaactacca aggagtttat taaaatcata gagagtgcag aaaatgagta
      841 tcagacagca attagtgaaa attaccaaac tatgtcagac accacgttca aggccttgcg
      901 ccggcagctt ccagttaccc gcaccaaaat cgactggaac aagatactca gttacaagat
      961 tggcaaagaa atgcagaatg ctagatctgc atggtccc
//
