LOCUS       sequence-159589-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of tagged protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  TITLE     Barr-Gillespie TwinStrep Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CAPZA1-TwinStrepII
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(41..64,65..100,101..124,125..130))
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
ORIGIN
        1 cggccggcca tcaccacttt gtacaagaaa gctgggttca cttttcgaac tgggggtggc
       61 tccaagcgga acctccactg ccccctccag atccgccccc cttctcaaat tgcggatggg
      121 accatgcaga tctagcattc tgcatttctt tgccaatctt gtaactgagt atcttgttcc
      181 agtcgatttt ggtgcgggta actggaagct gccggcgcaa ggccttgaac gtggtgtctg
      241 acatagtttg gtaattttca ctaattgctg tctgatactc attttctgca ctctctatga
      301 ttttaataaa ctccttggta gtttggattt cattagaaac agttactgag tcctgcacat
      361 ctttatgact aaccagctga acattgccat cttcataata atgaacctga atcttgagta
      421 ctccaaccac ctgggctgaa ggtggtgtaa tggtgaactt ccactctgat ctccagcgac
      481 cattccagaa gttttttggt tgaaactggt ggctttcaat acatgcaata atggtctgtt
      541 gcccatctat agttttagcg taaacagtac agaagccgtt ggaataatga tctttcacat
      601 aggctctcag agcactgtca cacgactccc tccaagactt caagcctcca tccacatcct
      661 ctggctgcgg gtcacttgct tctttccgta aatgatcaaa cttaaaggaa atttggtttc
      721 ttggatctaa aaatctgctg ttaccaaggt caccatgctc tgtaattaag acctggtcat
      781 cgtagccttc tatcttcacg ggtgtgaact gatccatgtt atactgggca aatgcatgtg
      841 ctgccccttc cctgaggaga ttgtcattgt taagcagtag ccggacatca ttgaatactt
      901 cattaaactc ccctgggggt gcatgggtga tgaatttagc ggctatgcgg accttctctt
      961 catccgacac ccga
//
