LOCUS       sequence-159590-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of tagged protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  TITLE     Barr-Gillespie TwinStrep Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CAPZB2-TwinStrepII
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..73
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     24..44
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    94..118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             950..973
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
       61 tataagcaga gctggtttag tgaaccgtca gatccaagtt tgtacaaaaa agcagggcgc
      121 caccatgagc gatcagcagc tggactgcgc cttggacctg atgaggcgcc tgcctccaca
      181 gcagattgag aagaacctca gcgatctgat cgacctggtc cccagtctgt gtgaagatct
      241 cctgtcatct gttgaccagc ccctgaaaat tgccagagac aaggtggtgg gcaaggatta
      301 ccttttgtgt gactacaaca gagacgggga ctcctatagg tcaccgtgga gtaacaagta
      361 tgaccctcct ttggaagatg gggccatgcc atctgctcgg ctcagaaagc tggaggtaga
      421 ggccaacaat gccttcgacc aataccgaga cctgtatttt gaaggtgggg tctcatcagt
      481 ctacctctgg gatcttgatc atggctttgc tggagtgatc ctcataaaga aagctggaga
      541 tggatccaag aagatcaaag gctgctggga ttccatccac gtggtggaag tgcaggagaa
      601 gtccagcggc cgtactgccc attacaagtt gacctccacg gtgatgctat ggctgcaaac
      661 caacaaatcc ggctcgggca ccatgaacct gggaggcagc ctaaccagac agatggagaa
      721 agacgaaact gtgagtgact gttccccaca catagccaac atcgggcgcc tggtggagga
      781 catggaaaac aaaatccgaa gcacgctgaa tgagatctac tttggaaaaa caaaggacat
      841 cgtcaacggg ctgaggtctg tgcagacgtt tgcagacaaa tcaaagcaag aagcgcttaa
      901 gaacgacctg gtggaggcct tgaagagaaa gcagcagtgt agatctgcat ggtcccatcc
      961 gcaatttgag aaggggggcg gatctggagg gggca
//
