LOCUS       sequence-159591-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of tagged protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  TITLE     Barr-Gillespie TwinStrep Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CAPZB2-TwinStrepII
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..40
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(44..67,68..103,104..127,128..133))
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     protein_bind    complement(959..983)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(1004..1048)
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 aagcggccgg ccatcaccac tttgtacaag aaagctgggt tcacttttcg aactgggggt
       61 ggctccaagc ggaacctcca ctgccccctc cagatccgcc ccccttctca aattgcggat
      121 gggaccatgc agatctacac tgctgctttc tcttcaaggc ctccaccagg tcgttcttaa
      181 gcgcttcttg ctttgatttg tctgcaaacg tctgcacaga cctcagcccg ttgacgatgt
      241 cctttgtttt tccaaagtag atctcattca gcgtgcttcg gattttgttt tccatgtcct
      301 ccaccaggcg cccgatgttg gctatgtgtg gggaacagtc actcacagtt tcgtctttct
      361 ccatctgtct ggttaggctg cctcccaggt tcatggtgcc cgagccggat ttgttggttt
      421 gcagccatag catcaccgtg gaggtcaact tgtaatgggc agtacggccg ctggacttct
      481 cctgcacttc caccacgtgg atggaatccc agcagccttt gatcttcttg gatccatctc
      541 cagctttctt tatgaggatc actccagcaa agccatgatc aagatcccag aggtagactg
      601 atgagacccc accttcaaaa tacaggtctc ggtattggtc gaaggcattg ttggcctcta
      661 cctccagctt tctgagccga gcagatggca tggccccatc ttccaaagga gggtcatact
      721 tgttactcca cggtgaccta taggagtccc cgtctctgtt gtagtcacac aaaaggtaat
      781 ccttgcccac caccttgtct ctggcaattt tcaggggctg gtcaacagat gacaggagat
      841 cttcacacag actggggacc aggtcgatca gatcgctgag gttcttctca atctgctgtg
      901 gaggcaggcg cctcatcagg tccaaggcgc agtccagctg ctgatcgctc atggtggcgc
      961 cctgcttttt tgtacaaact tggatctgac ggttcactaa accagctctg cttatataga
     1021 cctcccaccg tacacgccta ccgcccattt g
//
