LOCUS       sequence-159594-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  storage vector for BASIC part: GFP expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_10_J23101-RBS32-GFP-B0015
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(421..448)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(464..535)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tttaaacttt aaacatgtca aaagagacgt cttttgttaa gaatgctgag gaacttgcaa
       61 agcaaaaaat ggatgctatt aaccctgaac tttcttcaaa atttaaattt ttaataaaat
      121 tcctgtctca gtttcctgaa gcttgctcta aacctcgttc aaaaaaaatg cagaataaag
      181 ttggtcaaga ggaacatatt gaatatttag ctcgtagttt tcatgagagt cgattgccaa
      241 gaaaacccac gccacctaca acggttcctg atgaggtggt tagcatagtt cttaatataa
      301 gttttaatat acagcctgaa aatcttgaga gaataaaaga agaacatcga ttttccatgg
      361 cagctgagaa tattgtagga gatcttctag aaagatcgat aggtctcccg agcctataaa
      421 cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagagcg ttcaccgaca
      481 aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
      541 tggctctagt attattacgg acgcttatac agctcgtcca taccgtgggt gatgcccgca
      601 gcggtcacaa attccagcag gaccatgtga tcacgttttt cgttcggatc tttgctcagc
      661 acgctttggg tgctcagata gtgattatcc ggcagcaaaa ccggaccgtc accaattggg
      721 gtgttctgct ggtaatggtc ggccagttgg acgctaccgt cctcaacatt gtggcgaatc
      781 ttgaaattgg ccttgatgcc gttcttttgc ttgtctgccg taatgtaaac attatggctg
      841 ttgaagttat actccagttt atggcccaga atgttaccat cctctttaaa gtcgataccc
      901 ttcaattcga tacgattgac cagcgtgtc
//
