LOCUS       sequence-159982-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mTagBFP2-mEGFP-FRET
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..789
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     regulatory      814..823
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
       61 tcaagcttcg aattcaccat ggtgtctaag ggcgaagagc tgattaagga gaacatgcac
      121 atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc
      181 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct
      241 ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc
      301 aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg
      361 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
      421 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc
      481 cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct
      541 gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg
      601 atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct
      661 ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa cgagacctac
      721 gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa actggggcac
      781 aagcttaatt ccggactcag atccccaccg gtcgccacca tggtgagcaa gggcgaggag
      841 ctgttcaccg gggtggtgcc catcctggtc gagctggacg
//
