LOCUS       sequence-159994-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of tagged protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  TITLE     Barr-Gillespie TwinStrep Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SLC9A3R2-TwinStrepII
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        31..82
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     33..53
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    103..127
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      128..137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
       61 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccaagttt gtacaaaaaa
      121 gcagggcgcc accatggccg cgctggaatc gctgcggccc cgcttgtgtc gcctggtgcg
      181 cggagagcaa gggtacggct ttcacctgca tggcgagaaa ggccgccgtg gccagttcat
      241 ccggcgcgtg gagccaggct ccccagccga ggcggccgcg ctgcgcgccg gggaccgcct
      301 ggttgaggtc aacggcgtca acgtagaggg cgagacgcat caccaggtgg tacagaggat
      361 caaggctgtg gaggggcaga cccagctgct tgtggtggac aaggagaccg atgaggagct
      421 ctgccggcgg caactgacct gcactgagga gatggcccat cgagggcttc ccccagccca
      481 caacccctgg gagccaaagc cggactgggc atgctcgggc agccttggct cggacactgg
      541 ccagaaggat gtcaatgggc ccccaagaga gctgcgtccc cggctctgcc acttgcgaag
      601 gggaccccag ggctacgggt tcaacctgca tagtgacaag tcccggcctg gccagtacat
      661 ccgctctgtg gaccctggct ctcctgcttc tcactctggc ctccgtgccc aggatcggct
      721 cattgaggtg aatgggcaga acgtggaggg gctgcgacat gccgaggttg tcgccagaat
      781 caaggcacag gaggacgagg cccggctgct ggtagtcgat cctgagactg atgaacactt
      841 caagcggttg agggtcatac ccactgagga acatgtagaa ggtccactgc catcaccagt
      901 cacgaacggg accagtcctg cccaggatgg cagtacctgg aagcgagacc ccttccagga
      961 aagtggcctc cacctgagtc ccacagca
//
