LOCUS       sequence-159995-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of tagged protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  TITLE     Barr-Gillespie TwinStrep Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SLC9A3R2-TwinStrepII
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..41
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(45..68,69..104,105..128,129..134))
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
ORIGIN
        1 gaagcggccg gccatcacca ctttgtacaa gaaagctggg ttcacttttc gaactggggg
       61 tggctccaag cggaacctcc actgccccct ccagatccgc cccccttctc aaattgcgga
      121 tgggaccatg cagatctgaa gttgctgaag atctctcgct tccggttcca gtccatctgt
      181 ggtgcccgct tgttgacccg agtagctcga gccttctcct tggcctcagc tgctgtggga
      241 ctcaggtgga ggccactttc ctggaagggg tctcgcttcc aggtactgcc atcctgggca
      301 ggactggtcc cgttcgtgac tggtgatggc agtggacctt ctacatgttc ctcagtgggt
      361 atgaccctca accgcttgaa gtgttcatca gtctcaggat cgactaccag cagccgggcc
      421 tcgtcctcct gtgccttgat tctggcgaca acctcggcat gtcgcagccc ctccacgttc
      481 tgcccattca cctcaatgag ccgatcctgg gcacggaggc cagagtgaga agcaggagag
      541 ccagggtcca cagagcggat gtactggcca ggccgggact tgtcactatg caggttgaac
      601 ccgtagccct ggggtcccct tcgcaagtgg cagagccggg gacgcagctc tcttgggggc
      661 ccattgacat ccttctggcc agtgtccgag ccaaggctgc ccgagcatgc ccagtccggc
      721 tttggctccc aggggttgtg ggctggggga agccctcgat gggccatctc ctcagtgcag
      781 gtcagttgcc gccggcagag ctcctcatcg gtctccttgt ccaccacaag cagctgggtc
      841 tgcccctcca cagccttgat cctctgtacc acctggtgat gcgtctcgcc ctctacgttg
      901 acgccgt
//
