LOCUS       sequence-160005-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of tagged protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  TITLE     Barr-Gillespie TwinStrep Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: USH1CA1-TwinStrepII
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        32..83
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     34..54
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    104..128
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      129..138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
       61 gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccaagtt tgtacaaaaa
      121 agcagggcgc caccatggac cggaaggtgg cccgagaatt ccgacacaag gtggattttc
      181 tcattgaaaa tgacgcagag aaggactatc tttatgatgt gctgcggatg tatcaccaga
      241 ccatggatgt ggctgtgctc gtgggagacc tgaagctggt catcaatgaa cccaaccgcc
      301 tgccgctgtt tgatgccatt cgacccctga tcccactgaa gcaccaggtg gagtatgacc
      361 agctgacacc ccgacgctcc aggaaactga aggaggtacg cttggaccgt ctgcacccag
      421 aaggtctcgg cctcagcgtg cgtggaggcc tggaatttgg ctgtggactc tttatctccc
      481 acctcatcaa aggtggccag gcagacagcg ttgggcttca ggtaggggat gaaattgtcc
      541 ggatcaacgg ctattccatc tcttcctgta cccatgagga agtcatcaac ctgatccgca
      601 ccaagaagac cgtgtccatc aaagtgagac acatcggact gatccctgtg aagagctctc
      661 ctgaggagtc cctcaaatgg cagtatgtgg atcagttcgt gtcggaatct gggggtgtgc
      721 gaggtggctt gggctcacct ggcaatcgga caaccaagga gaagaaggtg tttatcagtc
      781 tagtgggctc tcggggcctg ggctgcagca tctccagtgg ccccatccag aagcctggca
      841 tcttcgtcag ccacgtgaag cctggctccc tgtctgcaga ggtggggtta gagacaggag
      901 accagattgt ggaagtcaac ggcatagact tcaccaacct ggaccacaag gaggctgtga
      961 atgtcctgaa gagcagccgc agcctgacca tctccatcgt tgctggagcc ggccgggagc
     1021 t
//
