LOCUS       sequence-160011-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of tagged protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  TITLE     Barr-Gillespie TwinStrep Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: USH1CA1-TwinStrepII
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..41
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(45..68,69..104,105..128,129..134))
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
ORIGIN
        1 gaagcggccg gccatcacca ctttgtacaa gaaagctggg ttcacttttc gaactggggg
       61 tggctccaag cggaacctcc actgccccct ccagatccgc cccccttctc aaattgcgga
      121 tgggaccatg cagatctaaa gaaggttagc tcgtcatcat actccttagg gggacagaca
      181 gcaaccacaa ggtctatcca gtccccgccc tgattccagg ccttctgtag ggcagcctcg
      241 gcctcagcca gggtgtagtc cgtcacaatc ttgccattga tggccatgat ctcatcccct
      301 ttcacaacgc caccatgcct ctccgcagct cccccttcat aaacagcaga gacgaccacc
      361 ttccccacgg gggagtccac gcctccctcc agggccaagt ctaaagaccc ctccttcttg
      421 attcgcagaa gccggacgtc cttccctgcg atctgctctg gcgagaacat ggagtaggga
      481 tcaaagcctt cttcgtattt tcgaaaatcc tgctgtctct gctcctccgt gcttccatcc
      541 aagtggtctg ccggctccac tccctgatca tacttgggct tgcgaagggg caccgggtgc
      601 acctctgcgg tgatggtctt aggcagaatg agctgctcct ttgaacccca gtcttctttc
      661 cactgcttct taaacttctc ttcctcctcc gagatctgtt ccatctcctt ccggtatctc
      721 tcattctcct cggcagcctt ctgggcgatc tcctttctcc tctggcgctc catctcctgc
      781 tgctcctgga ggatcttgtt ggactccatg gccagccgct tctgcatgag gagttcctgc
      841 cgttgcagct cacgctgccg tgcctcctcc agccgttccc ggtccgtcat gaacagctcc
      901 cggccggctc cagcaacgat ggagatggtc aggctgcggc tgctcttcag g
//
