LOCUS       sequence-160064-         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for Cas9 peptide array (linker length: 22aa)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Morita S, Noguchi H, Horii T, Nakabayashi K, Kimura M, Okamura K, 
            Sakai A, Nakashima H, Hata K, Nakashima K, Hatada I
  TITLE     Targeted DNA demethylation in vivo using dCas9-peptide repeat and 
            scFv-TET1 catalytic domain fusions.
  JOURNAL   Nat Biotechnol. 2016 Aug 29. doi: 10.1038/nbt.3658.
  PUBMED    27571369
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Aug 29. doi: 10.1038/nbt.3658."
COMMENT     Sequence Label: pCAG-dCas9-5xPlat2AflD
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..71
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             51..77
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             90..110
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             117..137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             201..257
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             324..380
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             447..503
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             570..626
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
ORIGIN
        1 tgtacgagac acggatcgac ctgtctcagc tgggaggcga cgcctatccc tatgacgtgc
       61 ccgattatgc cagcctgggc agcggctccc ccaagaaaaa acgcaaggtg gaagatccta
      121 agaaaaagcg gaaagtggac ggcattggta gtgggagcaa cggcagcagc ggatccaacg
      181 gtccgactga cgccgcggaa gaggagcttc tgagcaaaaa ctatcacctc gaaaacgagg
      241 ttgcgcgact gaagaaagga agcgggtccg gtggaagtgg ctccggatct ggaggttctg
      301 gcagcggagg tagcggcagt ggcgaagagc tccttagtaa gaactatcat ctggaaaatg
      361 aggtagcgcg cttaaagaaa gggtcgggaa gtggcggcag cggaagtggg agtggaggga
      421 gcggttctgg cggttccggc agtggagagg agttgctgtc taagaactac cacttagaaa
      481 acgaagtcgc acggctaaaa aaaggttccg gctccggcgg ctccggttct ggaagcgggg
      541 gctcgggatc aggtggatct ggatcaggag aggaattgct ttccaaaaac taccaccttg
      601 agaatgaggt ggccaggtta aagaagggga gcggctcggg gggtagtgga tcggggtcgg
      661 gcgggtcagg a
//
