LOCUS       sequence-160075-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP-dependent dGBP1-TagBFP, for expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Tang JC, Drokhlyansky E, Etemad B, Rudolph S, Guo B, Wang S, Ellis 
            EG, Li JZ, Cepko CL
  TITLE     Detection and manipulation of live antigen-expressing cells using 
            conditionally stable nanobodies.
  JOURNAL   Elife. 2016 May 20;5. pii: e15312. doi: 10.7554/eLife.15312.
  PUBMED    27205882
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-05-20"; volume: "5. pii: e15312. doi"; pages: " 
            10.7554/eLife.15312"
COMMENT     Sequence Label: pCAG-dGBP1-TagBFP
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..54
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     116..135
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      201..210
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
       61 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
      121 gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tctgcagtcg acggtaccgc
      181 gggcccggga tccaccggtc gccaccatgg ccgacgtgca gctcgtggaa tccggaggag
      241 cactggtcca gcctggggga tcactgagac tgtcttgtgt cgcttctggc tttcctgtga
      301 atcggtactc tatgagatgg tacaggcagg cacctggaaa agagcgagaa tgggtcgccg
      361 gaatgtcatc tgctggcgat aggagttcct acgtggatag cgtgaaagga cggttcacca
      421 ttagaaggga cgatgctaga aacaccgtgt acctccagat gaactccctg aaacctgagg
      481 acactgctgt gtactactac aacgtcaatg tgggctttga atactgggga catggcacac
      541 aagtgactgt tagttttgca tgcatgagcg agctgattaa ggagaacatg cacatgaagc
      601 tgtacatgga gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca
      661 agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct
      721 tcgccttcga catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca
      781 cccagggcat ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag
      841 tcaccacata cgaagacggg g
//
