LOCUS       sequence-160080-        5584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Van Antwerp DJ, Martin SJ, Kafri T, Green DR, Verma IM
  TITLE     Suppression of TNF-alpha-induced apoptosis by NF-kappaB.
  JOURNAL   Science. 1996 Nov 1. 274(5288):787-9.
  PUBMED    8864120
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1996 Nov 1. 274(5288):787-9."
COMMENT     Sequence Label: pCMX IkB alpha M
FEATURES             Location/Qualifiers
     source          1..5584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1170..1235
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1365..1385
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1657..1791
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1694..1713)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1748..1767
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2249..2383
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2500..2517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2671..3259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2751..2770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3430..4221,4222..4290))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4053..4072
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4291..4395)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4463..4481
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4519..4541)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4641..4660
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4805..5184
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5185..5388
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5339..5359
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5433..5452
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5433..5451
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgtttcagc cagctgggca cggccaggac tgggccatgg agggcccgcg ggatggcctc
       61 aagaaggagc gcttggtgga cgatcgccac gacgcaggtc tagacgccat gaaggacgag
      121 gagtacgagc aaatggtgaa ggagctgcgg gagatccgcc tgcagccgca ggaggcgccg
      181 ctggccgccg agccctggaa gcagcagctc acggaggacg gagactcgtt cctgcacttg
      241 gcaatcatcc acgaagagaa gccgctgacc atggaagtca ttggtcaggt gaagggagac
      301 ctggccttcc tcaacttcca gaacaacctg cagcagactc cactccactt ggctgtgatc
      361 accaaccagc caggaattgc tgaggcactt ctgaaagctg gctgtgatcc tgagctccga
      421 gactttcgag gaaatacccc tctacatctt gcctgtgagc agggctgcct ggccagtgta
      481 gcagtcttga cgcagacctg cacaccccag catctccact ccgtcctgca ggccaccaac
      541 tacaatggcc acacgtgtct gcacctagcc tctatccacg gctacctggc catcgtggag
      601 cacttggtga ctttgggtgc tgatgtcaac gctcaggagc cctgcaatgg ccggacagcc
      661 ctccaccttg cggtggacct gcagaatcct gacctggttt cgctcttgtt gaaatgtggg
      721 gctgatgtca acagggtaac ctaccaaggc tactccccct accagcttac ctggggccgc
      781 ccaagtaccc ggatacagca gcagctgggc cagctgacct tggaaaatct ccagatgcta
      841 cctgaggccg aggatgagga ggcctatgac gcggaggcag agttcgcaga ggatgagctg
      901 cccgatgatg actgtgtgtt tggaggccag cgtctgacat tataagtgga aagtggcaaa
      961 aagaatgtgg acttgtatat ttgtacaaat agagttttat ttttctaaaa aaaaaaaaaa
     1021 aaaggaattc gatatcaagc ttctcgaggg ggggcccggt accgatcctg gccagctagc
     1081 taggtagcta gaggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
     1141 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
     1201 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
     1261 gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat
     1321 ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa
     1381 aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt
     1441 ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc
     1501 tatacaagaa aattatggaa aaatatttga tgtatagtgc cttgactaga gatcataatc
     1561 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     1621 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     1681 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     1741 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat cagcttcaga
     1801 agatggcgga gggcctccaa cacagtaatt ttcctcccga ctcttaaaat agaaaatgtc
     1861 aagtcagtta agcaggaagt gactaactga ccgcagctgg ccgtgcgaca tcctctttta
     1921 attagttgct aggcaactgc cctccagagg gcagtgtggt tttgcaagag gaagcaaaaa
     1981 gcctctccac ccaggcctag aatgtttcca cccaatcatt actatgacaa cagctgtttt
     2041 ttttagtatt aagcagaggc cgggggcccc tggcctccgc ttactctgga gaaaaagaag
     2101 agaggcattg tagaggcttc cagaggcaac ttgtcaaaac aggactgctt ctatttctgt
     2161 cacactgtct ggccctgtca caaggtccag cacctccata ccccctttaa taagcagttt
     2221 gggaacgggt gcgggtctta ctccgcccat cccgccccta actccgccca gttccgccca
     2281 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     2341 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggctgcatta atgaatcggc
     2401 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctcat taatgaatcg gccaacgcgc
     2461 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2521 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2581 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2641 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2701 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2761 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2821 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2881 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2941 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3001 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3061 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3121 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3181 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3241 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3301 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3361 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3421 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3481 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3541 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3601 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3661 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3721 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3781 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3841 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3901 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3961 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4021 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4081 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4141 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4201 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4261 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4321 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4381 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     4441 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     4501 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     4561 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     4621 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     4681 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
     4741 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcg
     4801 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     4861 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     4921 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     4981 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     5041 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     5101 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     5161 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     5221 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     5281 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     5341 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
     5401 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
     5461 gctgtaccag atatcaggat cccccgggct gcaggaattc ccagcagcag cagcaacaac
     5521 agtcagacct cgccagccag ccgccagcag cctgcagcct gcacccgctc agccccgcac
     5581 agcc
//
