LOCUS       sequence-160092-         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Residues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable 
            N-term His-tag; Sundquist Lab Internal ID: WISP10-648.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
  TITLE     ALIX-CHMP4 interactions in the human ESCRT pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 
            10.1073/pnas.0801567105. Epub 2008 May 29.
  PUBMED    18511562
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0801567105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-06-3- 3"; volume: "105"; issue: "22"; pages: "7687-91"
COMMENT     Sequence Label: pet151D-TOPO ALIX Bro1-V (1-702)
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             44..85
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             86..106
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 actttaagaa ggagatatac atatgcatca tcaccatcac catggtaagc ctatccctaa
       61 ccctctcctc ggtctcgatt ctacggaaaa cctgtatttt cagggaattg atcccttcac
      121 ccatatggcg acattcatct cggtgcagct gaaaaagacc tcagaggtgg acctggccaa
      181 gccgctggtg aagttcatcc agcagactta cccaagcggc ggggaagagc aggcccagta
      241 ctgccgcgcg gcggaggagc tcagcaagct gcgccgcgcc gcagtcggtc gtccgctgga
      301 caagcacgag ggcgcgctcg agacgctcct gagatattat gatcagattt gttctattga
      361 acccaaattc ccattttctg aaaatcagat ctgcttgaca tttacctgga aggatgcttt
      421 cgataaaggt tcactttttg gaggctctgt aaaactggct cttgcaagct taggatatga
      481 aaagagctgt gtgttgttca attgtgcagc cttagctagc caaattgcag cagaacagaa
      541 cctggataat gatgaaggat tgaaaatcgc tgctaaacat taccagtttg ctagtggtgc
      601 ctttttacat attaaagaga cggttttatc tgccttaagt cgagagccga ccgtggacat
      661 atctccagat actgttggga ccctcagtct tattatgctg gcacaggctc aagaagtatt
      721 t
//
