LOCUS       sequence-160094-         260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Austin BP, Waugh DS
  TITLE     Isolation of Metarhizium anisopliae carboxypeptidase A with native 
            disulfide bonds from the cytosol of Escherichia coli BL21(DE3).
  JOURNAL   Protein Expr Purif. 2012 Mar;82(1):116-24. Epub 2011 Dec 14.
  PUBMED    22197595
REFERENCE   2  (bases 1 to 260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2012-03"; volume: "82(1)"; pages: "116-24. Epub
            2011 Dec 14"
COMMENT     Sequence Label: pBA2257
FEATURES             Location/Qualifiers
     source          1..260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attagcgagg atggtgatgg tgatggtgac tcatctgctg gaagagatgc atggcaccag
       61 caaagctctc ctctccagac ggtatgatct ggtccggtgg caggacaaac ccatacctac
      121 ctttgtcacg cagttcgatt gtgaagacat tgtcggcttt aagaacgtct tggacccaat
      181 caatagagcc accggcaact tggtagatga cattgcaaac tggaccatat gcgaaggtgg
      241 ttccgtggac actcctcata
//
