LOCUS       sequence-160097-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The catalytically inactive genetically encoded fluorescent H2O2 
            sensor roGFP2-Tsa2-Delta-CP-Delta-CR cloned in the yeast p415 vector
            under the control of a TEF promoter. For cytosolic expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Morgan B, Van Laer K, Owusu TN, Ezerina D, Pastor-Flores D, Amponsah
            PS, Tursch A, Dick TP
  TITLE     Real-time monitoring of basal H2O2 levels with peroxiredoxin-based 
            probes.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):437-43. doi: 10.1038/nchembio.2067. 
            Epub 2016 Apr 18.
  PUBMED    27089028
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2067"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "437-43"
COMMENT     Sequence Label: p415 TEF roGFP2‐Tsa2-Delta-CP-Delta-CR
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..72
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     56..72
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 acttttttta cttcttgctc attagaaaga aagcatagca atctaatcta agttttctag
       61 aactagtgga tccaccatgg ctagcgagtt ctcaaagggt gaagaattgt ttacaggtgt
      121 tgttcctatt ttagtcgaat tggacggtga cgttaatggt cataagttta gtgttagtgg
      181 tgaaggtgaa ggtgacgcaa catacggtaa attgaccttg aagtttattt caactactgg
      241 taaattgcca gttccttggc caactttggt aaccacttta acatatggtg tacaatgttt
      301 cagtagatac cctgatcata tgaaacaaca cgactttttc aagtctgcta tgccagaagg
      361 ttacgttcaa gaaagaacta ttttctttaa ggatgacggt aactacaaga ccagagcaga
      421 agtcaaattt gaaggtgaca ctttggttaa cagaatcgaa ttgaagggta tcgatttcaa
      481 ggaagacggt aacatcttgg gtcataaatt ggaatacaac tacaactgtc acaatgtata
      541 cataatggcc gataagcaaa agaatggtat caaagtcaac ttcaagatca gacataacat
      601 cgaagatggt tctgttcaat tagctgacca ctatcaacaa aatacaccta ttggtgacgg
      661 tcctgttttg ttaccagaca accattactt gtctacctgc tcagctttat ccaaagatcc
      721 aaatgaaaag agagaccata tggtattgtt agaatttgtc accgcagcag gtatcacatt
      781 gggtatggat gaattgtata a
//
