LOCUS       sequence-160102-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses fluorescent hybrid of tPA for PALM imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Scalettar BA, Shaver D, Kaech S, Lochner JE
  TITLE     Super-resolution imaging of neuronal dense-core vesicles.
  JOURNAL   J Vis Exp. 2014 Jul 2;(89). doi: 10.3791/51394.
  PUBMED    25046659
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2014-07-2"; volume: "(89). doi"; pages: " 
            10.3791/51394"
COMMENT     Sequence Label: tPA-Dendra2
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        247..265
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     247..264
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     278..297
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat
      121 tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc
      181 cacccccttg gcttcgttag aacgcggcta caattaatac ataaccttat gtatcataca
      241 catacgattt aggtgacact atagaataac atccactttg cctttctctc cacaggtgtc
      301 cactcccagg tccaactgca cctcggttct atcgattgcc accatgaagg gagagctgtt
      361 gtgcgtcctg ctgctttgtg gagtggcgtt cacgttgcct gaccagggaa tacacaggag
      421 gttcagaaga ggagctcggt cctacagagc gacctgcaga gatgaacaga ctcagacaac
      481 ttaccaacag catcagtcat ggctacgtcc catgctcaga ggcaatcggg tggaatactg
      541 ccggtgcaac agcggcctgg cacagtgcca ctcggtgccc gtccgaagtt gcagtgaacc
      601 gagatgcttc aatgggggta cgtgtcagca ggccctgtat ttctctgact tcgtctgcca
      661 gtgccctgac ggatttgttg ggaaacgctg tgatatagat accagagcaa cctgcttcga
      721 gggccagggc atcacctaca gaggcacatg gagcacagca gaaaatgggg ctgaatgcat
      781 caactggaat agcagtgccc tgtcccagaa gccctacagt gcaaggaggc caaatgccat
      841 caagctgggc cttgggaacc acaattactg cagaaaccca gaccgagacg tgaagccctg
      901 gtgctatgtc tttaaggcag ggaagtatac cacggagttc tgcagcacgc cggcttgccc
      961 taagggacca actgaggact gctatgt
//
