LOCUS       sequence-160103-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses fluorescent hybrid of tPA for PALM imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Scalettar BA, Shaver D, Kaech S, Lochner JE
  TITLE     Super-resolution imaging of neuronal dense-core vesicles.
  JOURNAL   J Vis Exp. 2014 Jul 2;(89). doi: 10.3791/51394.
  PUBMED    25046659
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2014-07-2"; volume: "(89). doi"; pages: " 
            10.3791/51394"
COMMENT     Sequence Label: tPA-Dendra2
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttataagctg caataaacaa gttgggccat ggcggccaag cttctgcagg tcgactctag
       61 atcaaccaca cctggctggg cagggggctg tagcgggcca cggcgtgctc gtacagcttc
      121 accttgttgt agtcgctgtc gttgcccagg atctcgatgc ggtggtccac gaagtgggcg
      181 tcgggcagct gcaccacctt cttggccttg taggtggtct tgaagtcgca caggtagtgg
      241 ccgccgccct ccagcagcag ggccatgttg atgttgccca ccagcaggcc gtcgcgcacg
      301 tgcagcttct cggtgctggg ctcccacttc agggtcttct tctgcatcac ggggccgttg
      361 ggggggaagt tggtgccctt gaagcgcacg ttctggaaga agcagtcgcc ctccaggctg
      421 atgtcgctgc ggatggtgca gatgcccttg tcctcgaagg tcatggtgcg ctcccagctg
      481 tagccctcgg ggaagctctg cttgaagtag tcggggatgt cctcggggta cttggtgaac
      541 acccggttgc cgtagtgcac ggcggtggtc aggatgtcgt agctgaaggg caggggggcg
      601 ccctccttca cggtcaggtt ggcggtctgg gtgccctcgt agggcttgcc cttgccctcg
      661 ccctcgatca cgaaggcgtg gccgttcacg ttgccctcca tgtgcacctt cacgcgcatg
      721 tcctccttga tcaggttacc ccgggatcct cctccacttc ctcctccttg cttcatgttg
      781 tcttggatcc agttcaggta attagtgacc tttgtgtata tccctggcac gtccttctgc
      841 ccacagccga ggccccagct gatgatgccc agtaaagtca tccgcttatc gatcatgcac
      901 accagagggc ctcctgagtc accctggcac gcgtcatgga cgtcttggtt gcccccagtt
      961 cgggtgtctc ctgcacacag catgttgctc gtgatggt
//
