LOCUS       sequence-160107-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Beta-catenin reporter. TCF/LEF sites upstream of a luciferase 
            reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Veeman MT, Slusarski DC, Kaykas A, Louie SH, Moon RT
  TITLE     Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, 
            regulates gastrulation movements.
  JOURNAL   Curr Biol. 2003 Apr 15. 13(8):680-5.
  PUBMED    12699626
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Apr 15. 13(8):680-5."
COMMENT     Sequence Label: M50 Super 8x TOPFlash
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        175..206
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      262..271
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(334..352)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tttctctatc gataggtacc gagctcttac gcgagatcaa agggggtaag atcaaagggg
       61 gtaagatcaa aggggcgcga gatcaaaggg ggtaagatca aagggggtaa gatcaaaggg
      121 ggtaagatca aaggggcgcg cccgcgtgct agcccgggct cgagatctag actctagagg
      181 gtatataatg gaagctcgaa ttccagcttg gcattccggt actgttggta aaaagcttgg
      241 cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa gaaaggcccg
      301 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag
      361 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc
      421 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
      481 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
      541 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
      601 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag
      661 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc
      721 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
      781 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca
      841 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct
      901 catagaactg cctgcgtgag attctcgcat gccagagatc ctatt
//
