LOCUS       sequence-160115-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP +787 from TSS; non-template strand; expressed 
            from aTc inducible system in pEZ03 in the NS1 locus with kanamycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas59
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaattcttca cacttttaac ggctgtctcc acagtgttcc tgactcctat ttacggagcg
       61 gctcaaacga tgcaagatca aagtaatacg atgaataatg aaaattcatt ggggaatagt
      121 gcgactgggg tagtcaacca aaccgctgtg tacaacttag gtcaaagtgc cacctacggt
      181 tttagaggga tcgtttgtcc ccgcccttcc tttgtgctca gtggctctgc aaatggcaat
      241 acatttgatg gggagagttc aaattcctac gctctttccg gtgccattat tgtgcccctc
      301 ggtggtcaag tgggcagcaa ctgtagagat ttcagcacta ccctactgga aagagaacaa
      361 gccgaattag caaagtttca aatccaatct tcagcggaaa cagtcaaact ttgtgctgac
      421 atgatttctt caggcattca agtagatcca gaggtttatc cagtactggc gcggcaatgt
      481 tctgcagttc ggttggtttc acaaggacgg gcttctagca caaatgaagc ccgacaaaat
      541 gaatcgactc acaataagct agatgatgat gtagaattgt ctgctaatta cttaaaaact
      601 gagtaaatat aatattttga atagtgagga aaaatatctc aaaggctata tgaaaataaa
      661 aaaagagcgg ctaaaaaatc ttcagcttac agtgacgcct gtctttgcat tctaaagcac
      721 taaaacacag tcgttttttt actgactcca agtgttttta ttactatact tagaaattcg
      781 tcaaataggc atgaaattgc tgtattaaaa tatcctactg tatcttttag ttcacactat
      841 ttttcttgag ttaattttgt atcaataa
//
