LOCUS       sequence-160121-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pACSA_J23113_tetR PEZ5_gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Zess EK, Begemann MB, Pfleger BF
  TITLE     Construction of new synthetic biology tools for the control of gene 
            expression in the cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   Biotechnol Bioeng. 2016 Feb;113(2):424-32. doi: 10.1002/bit.25713. 
            Epub 2015 Sep 3.
  PUBMED    26192329
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.25713";
            journalName: "Biotechnol Bioeng"; date: "2016-02"; volume: "113"; 
            issue: "2"; pages: "424-32"
COMMENT     Sequence Label: pEZ14
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             52..63
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(72..106)
                     /label=BBa_J23113
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.01"
ORIGIN
        1 ctctaatgcg ctgttaatca ctttactttt atctaatctg gacatctagt atttctcctc
       61 tttctctagt agctagcata atccctagga ctgagctagc catcaggtta acaaaaaagc
      121 aggaatatcc ctatcagtga tagacagaca tccctatcag tgatagagta taatgttaac
      181 tgtgggattg caaaaggagc tcccaactca taaagtcaag taggagatta atcatatgtc
      241 ggtcatcaag cccgaaatga aaattaaact gcgcatggag ggcgctgtga acgggcacaa
      301 gttcgtgatc gaaggtgaag gcattggtaa gccgtatgaa ggtactcaaa ccctggactt
      361 aacggttaaa gaaggtgccc cgttgccatt tagctacgac atcctgacac ccgcttttca
      421 gtatggcaat cgcgcgttca ccaagtatcc gaaagatatt ccggattatt tcaagcaggc
      481 attcccggaa ggatactcat gggaacgctc gatgacctac gaagaccagg gcatttgcat
      541 tgccacaagc gacatcacaa tggagggtga ttgctttttt tacaaaattc gcttcgacgg
      601 gacaaacttc ccgccgaatg gcccagtgat gcagaaaaag acgcttaaat gggaaccgtc
      661 gacagagaaa atgtacgttc gcgatggcgt tctgaaaggc gatgttaata tggcgctgct
      721 tctcgaaggg ggaggccatt accgttgtga tttcaaaacc acatacaaag caaaaaaaga
      781 tgttcgtttg ccggacgcgc atgaagtcga tcaccgcatt gagatcctgt cacatgataa
      841 agattacaat aaggtccgcc tttatgagca cgctgaggcc cgctattcta tgctgcccag
      901 ccaagctaaa taaggatccg gctgctaaca aag
//
