LOCUS       sequence-160127-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains pBAD-CusR (response regulator) and 
            pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy 
            tracking experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH003
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             411..476
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 aatcaccaga aattatgagc tcatacattc gtaagaaacg ggcgcaggag gattagatgg
       61 cagagtatgt gcgggccctg tttgacttta atgggaatga tgaagaagac cttcccttta
      121 agaaaggaga catcctgaga atccgggata agcctgaaga gcagtggtgg aatgcagagg
      181 acagcgaagg aaagaggggg atgattcctg tcccttacgt ggagaagtat ggtagtggca
      241 gcggcagcgg tagtctggaa atccgcgcag cgtttcttga aaaagaaaac accgcactgc
      301 gtacccgcgc ggccgaactc cgtaaacgtg tgggccggtg ccgcaacatt gtatccaaat
      361 acgaaacccg atacggcccg cttggttccg gttctggctc tggttccatg gactacaagg
      421 accatgacgg tgattacaag gatcatgaca tcgactacaa ggatgacgat gacaaggcaa
      481 ctagcggcat ggttagtaaa ggagaagaac ttttcactgg agttgtccca attttagttg
      541 aactagatgg cgacgtgaac ggtcataagt tcagtgtctc cggcgaaggt gagggtgatg
      601 caacgtacgg taagttaact ttgaagttaa tatgtacaac cggcaagctg cctgttccct
      661 ggcctaccct ggtgacaacg ttaggttatg ggttgatgtg ctttgctaga tacccagatc
      721 acatgaaaag gcatgacttc tttaaatctg caatgccaga aggttacgtc caagaacgta
      781 ctattttctt taaagatgac ggtaattata aaactagggc tgaagttaaa ttcgaaggtg
      841 acacacttgt aaatcgaata gagtt
//
