LOCUS       sequence-160131-         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop 
            codon (for native or N-terminal fusions), M72C mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Esposito
  TITLE     RAS mutant collection V2.0
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Hs.KRAS4b M72C
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             112..678
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYCRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(677..776)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cgaggatccg aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gataagcaat gcttttttat aatgccaact ttgtacaaaa aagttggcac catgactgaa
      121 tataaacttg tggtagttgg agctggtggc gtaggcaaga gtgccttgac gatacagcta
      181 attcagaatc attttgtgga cgaatatgat ccaacaatag aggattccta caggaagcaa
      241 gtagtaattg atggagaaac ctgtctcttg gatattctcg acacagcagg tcaagaggag
      301 tacagtgcaa tgagggacca gtactgcagg actggggagg gctttctttg tgtatttgcc
      361 ataaataata ctaaatcatt tgaagatatt caccattata gagaacaaat taaaagagtt
      421 aaggactctg aagatgtacc tatggtccta gtaggaaata aatgtgattt gccttctaga
      481 acagtagaca caaaacaggc tcaggactta gcaagaagtt atggaattcc ttttattgaa
      541 acatcagcaa agacaagaca gggtgttgat gatgccttct atacattagt tcgagaaatt
      601 cgaaaacata aagaaaagat gagcaaagat ggtaaaaaga agaaaaagaa gtcaaagaca
      661 aagtgtgtaa ttatgtaatc aactttcttg tacaaagttg gcattatagg aaagcattgc
      721 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgcatg
      781 caagcttaga tcctctagac cagcc
//
