LOCUS       sequence-160139-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 6xHis tagged PP3359 under pBAD regulation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Lo TM, Chng SH, Teo WS, Cho HS, Chang MW
  TITLE     A Two-Layer Gene Circuit for Decoupling Cell Growth from Metabolite 
            Production.
  JOURNAL   Cell Syst. 2016 Aug;3(2):133-43. doi: 10.1016/j.cels.2016.07.012.
  PUBMED    27559924
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.07"; journalName: "Cell Syst"; date: "2016-08";
            volume: "3"; issue: "2"; pages: "133-43"
COMMENT     Sequence Label: pBbE8k-PP3359-6xHis
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             23..34
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             509..526
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      544..615
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      631..658
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      697..768
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      784..811
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tttttgggaa ttcaagagat ctaaagagga gaaaggatct atggctaggt ctgcccgtag
       61 taccgacgac gcttgcgtgg ctgctcctgt gggagaaggg gtgcttgaag acttgatcgg
      121 ctacgccttg cgacgcgcgc aattgaagct gtttcagaac cttattgccc ggctctcggc
      181 ccatgacctg cgcccggccc aattttccgc cctggcgatc atcgaccaga accccgggct
      241 gatgcaggcc gacctggcgc gtgcgttggc aatcgacccc ccgcaagtcg tgccaatgct
      301 gaacaaactg gaagagcgcg cgctggccgt gcgcgtgcgg tgcaaaccgg acaagcgctc
      361 gtatgggatt ttcctgagca aatcgggcga ggccctgttg aaggagttga agcacatcgc
      421 cgccgacagc gatcaccagg cgacatccaa cctctcggat gacgaaagga ctgaactgtt
      481 gaggttattg aagaaaatct accgggacca tcatcatcat catcattaag gatctccagg
      541 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      601 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
      661 tataggatcc aaactcgagt aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg
      721 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
      781 actggctcac cttcgggtgg gcctttctgc gtttatacct agggcgttcg gctgcggcga
      841 gcggtatcag ctcactc
//
