LOCUS       sequence-160147-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses an shRNA targeting both S6K1 and S6K2 (mouse).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Hartman NW, Lin TV, Zhang L, Paquelet GE, Feliciano DM, Bordey A
  TITLE     mTORC1 targets the translational repressor 4E-BP2, but not S6 kinase
            1/2, to regulate neural stem cell self-renewal in vivo.
  JOURNAL   Cell Rep. 2013 Oct 31;5(2):433-44. doi: 
            10.1016/j.celrep.2013.09.017. Epub 2013 Oct 17.
  PUBMED    24139800
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.09.017"; journalName: "Cell Rep"; date: 
            "2013-10-31- 31"; volume: "5"; issue: "2"; pages: "433-44"
COMMENT     Sequence Label: pSicoR-shS6K1/2
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(37..70)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    126..714
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(179..199)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(597..608)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(717..733)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(718..734)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 acgagctgta caagtaggaa ttcgtcgagg gacctaataa cttcgtatag catacattat
       61 acgaagttat acatgtttaa gggttccggt tccactaggt acaattcgat atcaagctta
      121 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
      181 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
      241 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
      301 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
      361 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
      421 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
      481 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
      541 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
      601 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
      661 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
      721 accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
      781 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
      841 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
      901 tttttaaa
//
