LOCUS       sequence-160149-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses fluorescent hybrid of syntaxin-3 for PALM imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  TITLE     JPA5-Stx3-Dendra2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Stx3-Dendra2
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        247..265
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     247..264
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     278..297
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             922..933
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat
      121 tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc
      181 cacccccttg gcttcgttag aacgcggcta caattaatac ataaccttat gtatcataca
      241 catacgattt aggtgacact atagaataac atccactttg cctttctctc cacaggtgtc
      301 cactcccagg tccaactgca cctcggttct atcgatgcca ccatgaagga ccgactggag
      361 cagctgaagg ccaagcagct gacgcaggat gatgacacgg acgaggttga gattgctatt
      421 gataatacag cgttcatgga tgagttcttt tctgagattg aggaaacgag gctcaacatc
      481 gacaagatct cagagcatgt ggaggaagct aagaaactct acagtatcat tctctctgca
      541 ccgattccag agccaaaaac caaagacgac cttgaacagc tcacaactga gatcaagaaa
      601 agggccaaca acgtccggaa caaactgaag agcatggaga agcatattga ggaagacgag
      661 gtccggtcat cagcagacct tcggatacga aagtcccagc actccgtcct ctcccggaag
      721 tttgtggagg tgatgacaaa gtacaatgag gcacaggtgg acttccgtga acgcagcaaa
      781 gggcgcatcc aacggcagct tgaaattact ggcaagaaaa caacagatga ggagctggaa
      841 gaaatgttgg agagtggcaa cccagccatc ttcacttctg ggatcattga ctcccagatt
      901 tccaagcaag ccctcagcga gattgagggt cggcacaagg acatcgtgag gctggagagc
      961 agcatcaagg agctccatga catgt
//
