LOCUS       sequence-160151-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses a constitutively active mutant of 4E-BP1 under the CAG 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Hartman NW, Lin TV, Zhang L, Paquelet GE, Feliciano DM, Bordey A
  TITLE     mTORC1 targets the translational repressor 4E-BP2, but not S6 kinase
            1/2, to regulate neural stem cell self-renewal in vivo.
  JOURNAL   Cell Rep. 2013 Oct 31;5(2):433-44. doi: 
            10.1016/j.celrep.2013.09.017. Epub 2013 Oct 17.
  PUBMED    24139800
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.09.017"; journalName: "Cell Rep"; date: 
            "2013-10-31- 31"; volume: "5"; issue: "2"; pages: "433-44"
COMMENT     Sequence Label: pCAGGS-4EBP1 F113A
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(442..461)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(523..545)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ggcattggcc acaccagcca ccaccttctg ataggcagcc tgcacctgag gagtgaattc
       61 ttaaatgtcc atctcagctt gtgactcttc accgcctgcc cgcttatctt ccgggctgct
      121 gggcagttgg ctctggctgg cttgcatggg aggctcatcg ctggtagggc tagtgacccc
      181 aggaatggct ggcaggtcct ttgggggtgt tttggccaca ggtgagttcc gacactccat
      241 cagaaatttc cggtcataga taatcctggt tcctcccggg gtggtgctga agagcgtgcc
      301 gcccggagtg gtgctgtagt ccccgggcgg gagctgcacg ccatcgccga gggctacgcg
      361 gcgagtgggg atggcccggc tgggagtctg gctgcagctg ctgcccgccg acatgaattc
      421 tttgccaaaa tgatgagaca gcacaataac cagcacgttg cccaggagct gtaggaaaaa
      481 gaagaaggca tgaacatggt tagcagaggc tctagagccg ccggtcacac gccagaagcc
      541 gaaccccgcc ctgccccgtc ccccccgaag gcagccgtcc ccccgcggac agccccgagg
      601 ctggagaggg agaaggggac ggcggcgcgg cgacgcacga aggccctccc cgcccatttc
      661 cttcctgccg gcgccgcacc gcttcgcccc gcgcccgcta gagggggtgc ggcggcgcct
      721 cccagatttc ggctccgcac agatttggga caaaggaagt ccctgcgccc tctcgcacga
      781 ttaccataaa aggcaatggc tgcggctcgc cgcgcctcga cagccgccgg cgctccgggg
      841 gccgccgcgc c
//
