LOCUS       sequence-160152-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses a constitutively active mutant of 4E-BP1 under the CAG 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Hartman NW, Lin TV, Zhang L, Paquelet GE, Feliciano DM, Bordey A
  TITLE     mTORC1 targets the translational repressor 4E-BP2, but not S6 kinase
            1/2, to regulate neural stem cell self-renewal in vivo.
  JOURNAL   Cell Rep. 2013 Oct 31;5(2):433-44. doi: 
            10.1016/j.celrep.2013.09.017. Epub 2013 Oct 17.
  PUBMED    24139800
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.09.017"; journalName: "Cell Rep"; date: 
            "2013-10-31- 31"; volume: "5"; issue: "2"; pages: "433-44"
COMMENT     Sequence Label: pCAGGS-4EBP1 F113A
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(435..454)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    500..555
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(554..573)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(916..932)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(916..932)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tcggcgggca gcagctgcag ccagactccc agccgggcca tccccactcg ccgcgtagcc
       61 ctcggcgatg gcgtgcagct cccgcccggg gactacagca ccactccggg cggcacgctc
      121 ttcagcacca ccccgggagg aaccaggatt atctatgacc ggaaatttct gatggagtgt
      181 cggaactcac ctgtggccaa aacaccccca aaggacctgc cagccattcc tggggtcact
      241 agccctacca gcgatgagcc tcccatgcaa gccagccaga gccaactgcc cagcagcccg
      301 gaagataagc gggcaggcgg tgaagagtca caagctgaga tggacattta agaattcact
      361 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac
      421 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
      481 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
      541 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
      601 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
      661 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
      721 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
      781 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
      841 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag
      901 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaa
//
