LOCUS       sequence-160153-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For targeting shRNA construct into human AAVS1 locus, using genome 
            editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under
            U6 promoter, flanked by AAVS1 homology arms..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Daneshvar K, Pondick JV, Kim BM, Zhou C, York SR, Macklin JA, 
            Abualteen A, Tan B, Sigova AA, Marcho C, Tremblay KD, Mager J, Choi 
            MY, Mullen AC
  TITLE     DIGIT Is a Conserved Long Noncoding RNA that Regulates GSC 
            Expression to Control Definitive Endoderm Differentiation of 
            Embryonic Stem Cells.
  JOURNAL   Cell Rep. 2016 Oct 4;17(2):353-365. doi: 
            10.1016/j.celrep.2016.09.017.
  PUBMED    27705785
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-4- 4"; volume: "17"; issue: "2"; pages: "353-365"
COMMENT     Sequence Label: pAAVS1-shRNA
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    172..673
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     823..843
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 accagcattc tctcccctgg gcctgtgccg ctttctgtct gcagcttgtg gcctgggtca
       61 cctctacggc tggcccagat ccttccctgc cgcctccttc aggttccgtc ttcctccact
      121 ccctcttccc cttgctctct gctgtgttgc tgcccaagga tgctctttcc ggagcacttc
      181 cttctcggcg ctgcaccacg tgatgtcctc tgagcggatc ctccccgtgt ctgggtcctc
      241 tccgggcatc tctcctccct cacccaaccc catgccgtct tcactcgctg ggttcccttt
      301 tccttctcct tctggggcct gtgccatctc tcgtttctta ggatggcctt ctccgacgga
      361 tgtctccctt gcgtcccgcc tccccttctt gtaggcctgc atcatcaccg tttttctgga
      421 caaccccaaa gtaccccgtc tccctggctt tagccacctc tccatcctct tgctttcttt
      481 gcctggacac cccgttctcc tgtggattcg ggtcacctct cactcctttc atttgggcag
      541 ctcccctacc ccccttacct ctctagtctg tgctagctct tccagccccc tgtcatggca
      601 tcttccaggg gtccgagagc tcagctagtc ttcttcctcc aacccgggcc cctatgtcca
      661 cttcaggaca gcatgtttgc tgcctccagg gatcctgtgt ccccgagctg ggaccacctt
      721 atattcccag ggccggttaa tgtggctctg gttctgggta cttttatctg tcccctccac
      781 cccacagtgg ggcactagcc tcgagcggcc gcccccttca ccgagggcct atttcccatg
      841 attccttcat atttgcatat acgatacaag gctgttagag agataattgg aattaatttg
      901 actgtaaaca caaagatatt agtacaaa
//
