LOCUS       sequence-160154-        4931 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries operon 1 final
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Javidpour P, Deutsch S, Mutalik VK, Hillson NJ, Petzold CJ, Keasling
            JD, Beller HR
  TITLE     Investigation of Proposed Ladderane Biosynthetic Genes from Anammox 
            Bacteria by Heterologous Expression in E. coli.
  JOURNAL   PLoS One. 2016 Mar 14;11(3):e0151087. doi: 
            10.1371/journal.pone.0151087. eCollection 2016.
  PUBMED    26975050
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-14"; volume: "11(3):e0151087. doi"; pages: " 
            10.1371/journal.pone.0151087. eCollection 2016"
COMMENT     Sequence Label: pPJ174(JPUB_006779)
FEATURES             Location/Qualifiers
     source          1..4931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(16..33)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(150..695)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(230..249)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(809..903)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(934..1728)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1045..1064)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1655..1674
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     RBS             1959..1979
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      4790..4861
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4877..4904
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
       61 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
      121 ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat
      181 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
      241 gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
      301 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
      361 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
      421 cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc
      481 agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct
      541 aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag
      601 agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag
      661 agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa
      721 aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
      781 tacgatataa gttgttacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
      841 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
      901 gtccaagcga gctctcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
      961 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     1021 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     1081 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     1141 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg
     1201 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     1261 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     1321 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     1381 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     1441 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     1501 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag
     1561 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     1621 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     1681 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     1741 tcatcctgtc tcttgatcag atcatgatcc cctgcgccat cagatccttg gcggcaagaa
     1801 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
     1861 ttccgacgtc gaattcaaaa gatctcgtgc tttctatgag tcgttgctgc ataacttgac
     1921 atccctatca gtgatagata ggatgtgtgg aaataatttt gtttaacttt aagaaggagg
     1981 tatatccatg gctagcatga ctaaacatta tcgcattcat ctggagctga tttaatgaat
     2041 gatgaagaaa ttgagaaagg tgtgaccagt attgtggccg aagtgaccga actggatgaa
     2101 aaagaaatct gggaaaaacg tgatgccaac ttttttcaag acctggagat cgatagtctg
     2161 ctggccctgg aaattctggc gctgattgaa aagaagttca aagtgcaaat tccagaagaa
     2221 aaactggtgg atattaccag tctgagtgcc accattggtc tgaccaaaag tgttctggcc
     2281 gagagtggta aataaaggag gttttctaat ggataaacgt gtggttatta ccggtgtggg
     2341 tatgatcacg ccgattggca gcggtaagga tgtgttttgg aatgccctga ccgcgggtga
     2401 gagtggcgtg agcgaggtta gctgtattga caccagtggt taccgcgtgc ataaaggttg
     2461 tgaagttaag aactttaact acagtgacta catcaaaaat ggtgtgctga aggaaattgg
     2521 taaaggtagt cagtttgcga ttgccgcgac gaaactggcg ctggatgatg ccaagatgga
     2581 tctgagtaaa attgaactgg aacgtatggg tgtgagtgtt ggtacgaccg ccggtgaaat
     2641 tcaaattctg gaaaaggtga attacattcg tcatcgtgat ggcgaggaca aagttgcccc
     2701 ggacctgttc ctgatgcacc cgtgcaacaa catcccggcc aacattgcca tcgagtttgg
     2761 cctgaagggc ccaaatacga ttattccaac cgcctgtgcg gcgggtaact acgcgatcgg
     2821 ttatgcctac gatctgattc gttttggccg cgttgatgtt atggtggccg gtggcagtga
     2881 tccatttagt aaggtggcgt ttacgggttt tgcccgtctg ggcgccattg ccccagaaat
     2941 ttgtcaacca tttgataaaa accgtaaggg catgatggtg ggcgaaggtg cgggcatgct
     3001 ggtgctggaa agtctggaga gtgcggaaaa acgtaacgcc aatgtgtacg ccgagattat
     3061 tggttacggt ctgagttgtg acgcctacca tattaccatc ccacatccag atggtgaagg
     3121 tgtgattagt gccatgatca aagccctgaa aagcgccaat ctgcgtccag aagacgtgca
     3181 atacattagc gcccatggta ccggcacgcc agccaatgat aaagcggaaa ccattagtat
     3241 taagaaagtg tttggtgaaa aaccagagca tctggccatc agcagtatca aaagtatgat
     3301 tggtcatacg atgggtgccg ccagtgttat tgaagccatc gcgtgcgccc tggccgtgca
     3361 gaacgacatc attccaccga ccatcaatta cgttacccca gacccagaat gtgacctgga
     3421 ctacgtgcca aatgtgatgc gcaagcagga agtgaatatt gcgctgaaca atgcccatgc
     3481 ctttggtggc aacaatagtt gtctggtgat caagaagttc acgaactaaa ggaggttttc
     3541 taatgggcga cacgcgtatt gttatcaccg gtatcagtgt gattagccca ctgggtgaaa
     3601 ccaaagaaga cttttggcaa aatctgatca acggtgtgag tggtattaaa ccagttaccc
     3661 tgtttgatgt tacccacttt aatagtaaac aagccggtga gattaccgat tttgacccaa
     3721 agatttacct gggtaaaaaa ggtatccgtc atatcgatcg taccagtctg ctggtgagta
     3781 gcgcgacctg tctggcgatt aaagacgcca atctggaaaa caacacctac aatgaggatg
     3841 agctgggtat cgtggttggt agtacgtatg gtagcatcga tagtattagc agttttgatt
     3901 tccaaagtct gcgcgagggt ccaaattacg tgaatccaat ggattttccg aataccgtga
     3961 tcaatgcgcc agccagtcgc gccagtatct tttgcaaggc cacgggtctg agtaccacca
     4021 ttagcaatgg tgttacgagc agtatcgacg cgatcattta cgcgagtgac tttctgcgca
     4081 tgggtcgtgt gaaagccgtt attgccggtg gcgtttacgg tctgggtcac gacattttct
     4141 ggggtgcgca tagtagtaaa attctggccg gcagtaatag cggtaccctg gaaattagcg
     4201 ccccatttga taaacgtcgt aatggtatgg ttctgggcga ggccagcgcc ctggtgatcc
     4261 tggagacgct ggaagatgcg ctgaaacgta atgcgaacat ttatgccgag gtgcgcggtt
     4321 acggtacggc ctttgatccg aaaatgagta cgagtaaaga atatcaaacg gacggcagca
     4381 tccgcgccat tcgtagtgcg ctggaggatg ccaagctgac gctgcatgat attagttgta
     4441 ttagtgcgaa tgcctacagt ggcgtgtatg gtgatgccat ggaggttcgt gccctgaagg
     4501 aagtttttgg tatgcgtgcc aaactgattc cggtgaccgc catcaaaagt atgaccggcg
     4561 aatgtctgga tgcgagtggc gcgctgcaaa ccgttgcggc ggtgatgagc ctgaacaccc
     4621 atacgattcc gccgaccatt aactacaaag aaccagatcc agaatgtgac ctgggcctgg
     4681 tggtggagcg tgcggcgacg gtgccagtga aaaatgtgct gattaacagc agcagtcgcc
     4741 tgggcaatag tagcagtctg attgtgagta aattcaaata accaggcatc aaataaaacg
     4801 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     4861 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata ggatcctaaa
     4921 agcttcctag g
//
